Antibodies

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Aldolase A / ALDOA antibody

IgG purified antibody to rabbit muscle aldolase (R1054, R1054P and R1054HRP) was used at a 1/1000 dilution to detect human aldolase by western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4° C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE). Other detection methods will yield similar results. This antibody clearly detects a band at ~41 kDa consistent with human aldolase. Goat HRP Hu, Rb E, IP, WB
0.1 mg / $515.00
  OriGene Technologies GmbH

Aldolase A / ALDOA antibody

IgG purified antibody to rabbit muscle aldolase (R1054, R1054P and R1054HRP) was used at a 1/1000 dilution to detect human aldolase by western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4° C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE). Other detection methods will yield similar results. This antibody clearly detects a band at ~41 kDa consistent with human aldolase. Goat Purified Hu, Rb E, IP, WB
0.1 mg / $515.00
  OriGene Technologies GmbH

Alpha-2-macroglobulin / A2M antibody

  Goat polyclonal Alpha-2-Macroglobulin antibody  
Goat anti Alpha-2-Macroglobulin (200-101-207 lot 20172) was used to detect Alpha-2-Macroglobulin under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 649 conjugated Donkey anti goat. Goat Serum Hu E, IP, WB
2 ml / $1,345.00
  OriGene Technologies, Inc.

ARIH2 / TRIAD1 (C-term) antibody

  Goat Anti-ARIH2 / TRIAD1 Antibody  
TA305751 (2µg/ml) staining of Hela (A) and K562 (B) nuclear lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt ICC/IF, IP, WB
0.1 mg / $360.00
  OriGene Technologies, Inc.

Arrestin beta-2 / ARRB2 antibody

  Goat Polyclonal Antibody against ARRB2  
TA303022 (0.03µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt E, IP, P, WB
0.1 mg / $360.00
  OriGene Technologies, Inc.

ASS1 antibody

  Goat Polyclonal Antibody against Argininosuccinate synthetase 1  
TA302415 (0.03µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, ICC/IF, IP, P, WB
0.1 mg / $360.00
  OriGene Technologies, Inc.

ASS1 (196-212) antibody

Immunohistochemistry Image: Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bov, Bt, Can, Elephant, Eq, Hu, Mky, Ms, Por, Rt E, IP, P, WB
50 µg / $425.00
  OriGene Technologies GmbH

BICC1 (223-234) antibody

  Goat Anti-BICC1 (aa223-234) Antibody  
HEK293 overexpressing Human BICC1 with N-terminal DDK (FLAG) tag, probed with rabbit anti-BCC1 in Western Blot after IP using either TA311176 or anti-DDK antibody (Cat# TA50011-100) in the presence or absence of SDS. Lane 1: native, TA311176; Lane 2: native, anti-DDK (FLAG); Lane 3: SDS, TA311176; Lane 4: SDS, anti-DDK (FLAG). Goat Aff - Purified Can, Hu, Por, Rt E, IP
0.1 mg / $360.00
  OriGene Technologies, Inc.

Carbonic anhydrase 1 antibody

Western blot of goat anti Carbonic anhydrase I antibody Cat.-No. R1067P. Lane 1: Carbonic anhydrase I. Load: 50 ng per lane. Primary antibody: Carbonic anhydrase I antibody at 1:1,000 overnight at 4°C. Secondary antibody: HRP goat secondary antibody at 1/40,000 for 30 min at RT. Blocking buffer for 30 min at RT. Predicted/Observed size: 29 kDa, 29 kDa for Carbonic anhydrase I. Goat Purified Hu E, IP, WB
0.1 mg / $515.00
  OriGene Technologies GmbH

CD244 pTyr271 antibody

  Goat Anti-phospho-CD244 / 2B4 Antibody  
(2ug/ml) staining of IP from a lysate of Human Natural Killer cells with (+) or without (-) pervanadate treatment of the cells. Primary incubation was 1 hour. Detected by chemiluminescence. Data kindly provided by Dr Nicholas G Clarkson, Sir William Dunn School of Pathology, Oxford, UK Goat Aff - Purified Hu E, IP, WB
0.1 mg / $360.00
  OriGene Technologies, Inc.

Fibrinogen antibody

  Goat polyclonal anti-Fibrinogen antibody  
Western Blot of Goat anti-Fibrinogen antibody. Lane 1: Fibrinogen under reducing conditions. Lane 2: none. Load: 1 µg per lane. Primary antibody: Fibrinogen antibody at 1:3000 for overnight at 4°C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 45 min at RT. Block: TBS/MB-070 1 hr RT. Goat Serum Hu E, IP, P, WB
2 ml / $1,345.00
  OriGene Technologies, Inc.

HSP60 antibody

Human Uterus (formalin-fixed, paraffin-embedded) stained with HSPD1 antibody AP22882PU-N followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Purified Bac, Bov, Can, Dros, Fi, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh, Xen IP, P, WB
50 µg / $425.00
  OriGene Technologies GmbH

IRF5 (C-term) antibody

  Goat Polyclonal Antibody against IRF5  
TA302667 (0.1ug/ml) staining of lysate of cell line A549 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Rt ChIP, E, IP, P, WB
0.1 mg / $360.00
  OriGene Technologies, Inc.

KPNA3 / Importin alpha-3 (C-term) antibody

  Goat Polyclonal Antibody against KPNA3 / IPOA4  
TA302524 staining (0.1µg/ml) of Human Testis lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, ICC/IF, IP, P, WB
0.1 mg / $360.00
  OriGene Technologies, Inc.

MAPK6 / ERK3 antibody

HEK293 lysate (10 µg protein in RIPA buffer) overexpressing GFP-fused Mouse ERK3 probed with MAPK6 / ERK3 Antibody Cat.-No AP32807PU-N (0.5 µg/ml) in left panel and with anti-GFP in right panel. GFP-only exression in the first lane. Primary incubations were for 2 hours. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, IP, P, WB
0.1 mg / $380.00
  OriGene Technologies GmbH

MAPKAP Kinase-2 antibody

AP32810PU-N  (0.5 µg/ml) staining of MEF lysates (35µg protein in RIPA buffer), from double KO mice in second and third lanes and rescued by introduction of MK2 gene in second lane. Primary incubation was 2 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms E, IP, P, WB
0.1 mg / $380.00
  OriGene Technologies GmbH

MAPKAP Kinase-2 (44-55) antibody

HEK293 overexpressing Mouse MK2 fused to GFP or overexpressing MK5 fused to GFP and probed with AP32810PUN (0.5 μg/ml) in the left panel and with AP32811PU-N (0.5 μg/ml) in the right panel. Goat Aff - Purified Hu, Ms, Rt E, IP, WB
0.1 mg / $380.00
  OriGene Technologies GmbH

MAPKAP Kinase-5 antibody

AP32808PU-N (5 µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, IP, P, WB
0.1 mg / $380.00
  OriGene Technologies GmbH

MAPKAP Kinase-5 (192-206) antibody

AP32809PU-N (5 µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, IP, P, WB
0.1 mg / $380.00
  OriGene Technologies GmbH

NPY Receptor 5 / NPY5R antibody

  Goat Polyclonal Antibody against NPY5R  
TA303031 (0.3µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, IP, P, WB
0.1 mg / $360.00
  OriGene Technologies, Inc.

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